邹文安
一、上传数据
1/ 准备所用数据：
从Maxquant软件搜库原始数据combined\andromeda中下载.apl文件，压缩为.zip格式；同时，从combined\txt文件中选择msms.txt文件。
2/ 打开网址，准备上传。按要求导入apl文件和txt文件后，设置参数：
http://msviewer.ucsf.edu/prospector/cgi-bin/msform.cgi?form=msviewer
Results File Format：Maxquant
Constant Mods： Carbanidomethyl（C）
Frag Tol：0.02
Instrument： ESI-Q-high-res
3/ 点击“Upload New Results”；等待上传；
点击“ Display and Parameter Settings”前面的“+”号，展开
“Save settings”—“Rgenerate Report”。
上传成功后保存好给出的信息。
二、查看数据、谱图
1/ 登陆http://msviewer.ucsf.edu/prospector/cgi-bin/msform.cgi?form=msviewer
2/ 输入上面获得的Search Key：点击“Get Existing Results”
3/ 从结果中选择肽段，点击即可得到谱图结果。
注明：A more amenable alternative to fulfill this requirement (especially for large datasets) is through deposition of spectra to MS-Viewer (http://msviewer.ucsf.edu/prospector/cgi-bin/msform.cgi?form=msviewer). You can simply upload the .apl files (zipped together) and msms.txt files from the MaxQuant analysis output. Upon successful upload, a unique search key will be generated, which will need to be included in the manuscript.